Exome Sequencing

For AmpliSeq Exome analysis a minimum of 1 ml of blood or 200 ng of high molecular weight DNA is recommended to ensure excellent quality sequencing data. Completely annotated data in excel files and/or raw sequence reads (bam or fastq format) will be provided. The cost per exome ranges between $700 and $1,350 depending on the number of samples, source material and bioinformatics analysis requested. For additional information or a quote please visit our NGS services page.

Exome certified logo

DNA Requirements

  • Quantity: 50-200 ng
  • Concentration: ≥ 10 ng/μl
  • OD260/280 = 1.8-2.0

Sequencing Metrics

  • Throughput:  >5 Gb
  • Read length: 150-200 bp
  • Coverage: >100x

Timeline and Price

  • Standard processing time for less than 10 exomes is under 4 weeks
  • Starting at $700 per exome

Transcriptome Analysis

Ion AmpliSeq Transcriptome solution is specifically designed for gene-level expression analysis of human or mouse samples. The Ion AmpliSeq Transcriptome Gene Expression Kit targets >20,000 RefSeq transcripts using as little as 10 ng RNA input. The cost of AmpliSeq transcriptome is between $300 and $400 depending on the number of samples processed.

Whole Transcriptome sequencing provides an unbiased output with a greater quantitative linear dynamic range and improved detection of novel transcripts and alternatively spliced isoforms. For transcriptome analysis a minimum of 1 μg of total RNA is recommended to generate high quality reads. The cost of transcriptome sequencing ranges between $450 and $1,300 depending on the number of samples, sequencing reads (10-80 million) and bioinformatic analysis.

For additional information or a quote please visit our NGS services page.

Ion proton chip

Target Panels

AmpliSeq Ready-to-use and Custom panels for RNA and DNA are also available, those include:

DNA Panels

  • Inherited diseases
  • Pharmacogenomics
  • Cancer
  • Ancestry
  • To see all available DNA panels click here

RNA Panels

  • Cancer
  • Fusion Lung Cancer
  • Apoptosis